Effects of meiosis-activating sterol on rRNA gene transcription and the developmental potential of preimplantation porcine embryos

目前的体外生产系统对产生的胚胎的正常影响正在恶化。编码拓扑异构酶I、聚合酶I、上游结合因子、纤维蛋白、核仁蛋白和核磷蛋白的基因偏离正常激活可能参与了这种影响。利用免疫细胞化学和RT-PCR技术，我们将研究体内胚胎rRNA转录重新启动的分子机制，从而开发合适的标记物来评估在各种体外条件下产生的胚胎的正常性。特别强调将致力于减数分裂激活甾醇（MAS）在体外猪胚胎早期发育中的作用。卵母细胞培养条件的改进将重点放在改善体外猪胚胎生产系统，使其能够在常规基础上产生具有足够活力的胚胎。这些数据将有助于了解猪着床前胚胎发育的细胞和分子调控机制，增加胚胎正常程度评价指标的数量，改善胚胎的培养条件，最终提高胚胎在生物医学研究中的正常程度。

Current in vitro production systems have deteriorating effects on the normality of the resulting embryos. Deviations from the normal activation of the genes encoding the Topoisomerase I, Polymerase I, Upstream binding factor, Fibrilarin, Nucleolin and Nucleophosmin may be involved in this effect. Employing immunocytochemistry and RT-PCR technology we will study the molecular mechanisms involved in re-initiation of the embryonic rRNA transcription in vivo and thus develop suitable markers for evaluation of the normality of the embryos produced under various in vitro conditions. Special emphasis will be devoted to the role of meiosis activating sterol (MAS) during early development of the porcine embryo in vitro. Modification of oocyte culture conditions will focus on improvement of the in vitro pig embryo production system to such a level that the embryos of a sufficient viability can be produced on a routine basis. These data will contribute to understanding of the cell and molecular mechanisms regulating porcine pre-implantation embryonic development, increase the number of markers suitable for evaluation of the normality of the embryos, improve the culture conditions and finally increase the normality of embryos used in biomedical research.