Identification of protein dynamics for enzymatic reaction using four-dimensional visualizing analysis

本研究的目的是建立酶促反应机理中蛋白质动力学的可视化方法。在这种情况下，我们选择了含铜亚硝酸还原酶的晶体作为测试案例，并收集了高亮度X射线诱导还原过程中的衍射数据。因此，我们成功地观察到一些明确的结构变化，在铜结合位点的差异傅立叶电子密度图。

The objective for this research project is to establish the methods for visualizing the protein dynamics on enzymatic reaction mechanism. In this case, we selected a crystal for copper-containing nitrite reductase as a test case, and collected the diffraction data during high-brilliant x-ray induced reduction. As a result, we have succeeded in observation of some unambiguous structural changes at the copper binding sites in the difference fourier electron density maps.