Method Development for Assessing the Progression of Metabolic Syndrome by Multiplex-dynamic Analysis of Compounds of Biological Importance

建立了代谢综合征的多重分析方法，用于检测代谢综合征的进展。采用灵敏、精确的高效液相色谱电化学检测法，对疾病动物模型中的血浆游离脂肪酸、胆固醇和病理学上重要的固醇进行动态分析。此外，一种新的分析策略的基础上统计方面被证明是适用于检查之间的相互作用的游离脂肪酸和血糖水平。结果表明，本方法可用于临床各种动力学研究。

The multiplex analytical methods for determining compounds of biological importance were developed to examine the progression of metabolic syndrome. Dynamic analyses of plasma free fatty acids, cholesterol, and pathologically important sterols in animal model of disease were performed by sensitive and precise HPLC with electrochemical detection. Moreover, a novel analytical strategy based on statistics aspect was shown to be applicable to examine the interaction between free fatty acids and blood glucose level. It was concluded that the present methods were useful various kinetic studies in clinical fields.