Fabrication of anodized titanium artificial bone induced osteoblast cells

采用光照射图案化方法在钛板上制备了微米级图案化二氧化钛薄膜。在含HAp的碱性槽中，采用恒流密度、直流或脉冲电流密度对钛表面的图案化二氧化钛凝胶膜进行电火花氧化，从而在图案化的二氧化钛膜上制备出不同形状的孔。此外，除刻蚀工艺外，还采用光敏化和阳极氧化相结合的方法制备了反转图案化薄膜。细胞培养检测表明，与未加羟基磷灰石的氧化钛膜相比，负载羟基磷灰石的二氧化钛薄膜的生物活性有了很大的提高。

Micrometer-scale patterning-TiO_2 films derived by sol-gel method were formed on a titanium plate using by photo-irradiation patterning method. The patterned TiO_2 gel film onto titanium was then anodized at constant current density with DC or pulsed-current density by anodic oxidation in an HAp-containing alkaline bath under spark discharge, to follow preparing various pore shapes on patterned-TiO_2 film. Moreover, reversed patterning-film was also prepared by the combination of photosensitizing and anodizing method except etching process. The bioactivity, which was investigated by cell culture examination, was drastically improved for HAp-loaded titania film in comparison with anodized titanium film without HAp.