Analysis of physicochemical properties and functions of quinone molecule in the homodimeric photosynthetic reaction center

在绿色硫磺细菌中构建了一个新的分子生物学系统，该系统人为地将光合作用反应中心(RC)的同源二聚体结构改变为异二聚体结构。通过使用Ni-NTA和Strep-Tactin树脂的串联层析过程，成功地获得了Strep-PSCA/His-PSCA-型RC。还开发了用于大宿主范围质粒转化系统的方法，以简化遗传分析，并将在不久的将来成为有用的工具。

The novel molecular biological system, which artificially alter the homodimeric structure of the photosynthetic reaction center(RC) to the heterodimeric one, has been constructed in the green sulfur bacterium, Chlorobaculum tepidum. By the tandem chromatographic procedures using Ni-NTA and Strep-tactin resins, the Strep-PscA/ His-PscA-type RC could be obtained successfully. The method for the transformation system with a broad-host-range plasmid has also been developed to simplify genetic analyses, and will be a useful tool in the near future.