A study on the starvation response mechanism of Rickettsia, an obligate intracellular parasitic bacteria

我们制备了针对硬蜱自噬相关抗原Atg12的抗体，以监测硬蜱细胞饥饿状态的建立，以阐明立克次体在饥饿环境中生存的反应机制，立克次体节肢动物载体。非致病性斑点热群立克次体感染蜱细胞和哺乳动物细胞时，立克次体的生长受到抑制。自噬被认为是造成这种限制的原因。此外，当感染细胞被致病立克次体重叠感染时，非致病立克次体的生长也被诱导。我们推测致病立克次体产生自噬的限制因子(S)的可能性。

We generated antibody to autophagy-related antigen Atg12 of a tick to monitor the establishment of starvation state on tick cells to elucidate the response mechanism for rickettsiae to survive in an environment of starvation in ticks, arthropod vectors of rickettsiae. When tick cells and mammalian cells were infected with nonpathogenic spotted fever group rickettsiae, the growth of rickettsiae was restricted. Autophagy was suggested to be contributed to this restriction. Moreover, when the infected cells were superinfected with pathogenic rickettsiae, the growth of the nonpathogenic rickettsiae were induced. We speculated the possibility that the pathogenic rickettsiae generate a restriction factor(s) of autophagy.