Analysis of a role for BCR-ABL1 in the leukemogenesis using the experimental system that reflects the characteristics of human leukemia

我们设计了一种独特的培养系统，可以同时评估来自人造血干细胞的B细胞和T细胞以及骨髓细胞的发育。bcr - abl1转染的人CD34^+细胞具有与慢性骨髓性白血病细胞不同的分化能力。外周血中的单核细胞被证明是造血干细胞来源的肝星状细胞的直接前体。结果表明，在急性型成人T细胞白血病/淋巴瘤中，淋巴结中存在多个共同克隆起源的亚克隆，并选择一个亚克隆进入外周血。

We devised a unique culture system in which the developments of B cells and T cells as well as myeloid cells from human hematopoietic stem cells can be simultaneously evaluated. BCR-ABL1-transfected human CD34^+ cells showed the highly proliferative potential with different differentiation capacity from that of chronic myelogenous leukemia-derived cells. Monocytes in the peripheral blood proved to be immediate precursors of hepatic stellate cells of hematopoietic stem cell origin. It turned out that in acute type adult T cell leukemia/lymphoma, multiple subclones of common clone origin are present in the lymph nodes and a selected subclone exits into the peripheral blood.