Isolation of adult pancreatic stem cells for regenerative medicine of diabetes mellitus

虽然胰腺器官移植是I型糖尿病唯一可治愈的选择，但可移植的供体组织极度短缺。组织特异性干细胞在糖尿病的再生医学中具有独特的应用价值。为此，我们制作了泛干细胞标记（CD133）敲除/GFP敲除小鼠。在CD133^（+/GFP）小鼠中，GFP阳性细胞集中在胰腺、肝脏和肠道等特定区域的细胞，这被认为是干细胞生态位。

Although pancreatic organ transplantation is only curable option in type I diabetes mellitus, there is an extreme shortage of engraftable donor tissue. Tissue-specific stem cells are uniquely suited for regenerative medicine of diabetes mellitus. To this end, we produced pan-stem cell marker(CD133) knockout/GFP knockin mice. In CD133^(+/GFP) mice, GFP positive cells concentrated on the cells in specific region such as pancreas, liver and intestine, which is thought to be stem cell niche.