Development of novel functional oligonucleotide analogs for detection and regulation of a single base alteration in gene

基因的先天性和获得性碱基突变与各种疾病和个体体质有关。本研究合成了新的功能核酸类似物，用于开发一种新的检测基因单点突变和抑制癌细胞增殖的系统。获得了以下结果：(1)用于基因诊断和基因调控的新型功能核酸类似物的合成；(2)RUNX1和JAK2基因碱基改变的均相荧光检测方法的建立；(3)光动力反义技术选择性抑制癌细胞增殖；(4)活细胞RNA成像方法的建立。通过这项研究，我们相信具有复杂功能的核酸可以揭示未知的“RNA”功能。

The congenital and acquired base mutations in genes are related to various diseases and to individual physical constitution. In this research, novel functional nucleic acid analogs were synthesized for development of a novel detection system for a single point mutation in gene and for suppression of cancer cell proliferation. Following results were obtained ;(1) Synthesis of novel functional nucleic acid analogs for gene diagnosis and gene regulation ;(2) Development of a protocol for homogeneous fluorescent assay of base alteration in RUNX1 and JAK2 genes ;(3) Selective inhibition of cancer cell proliferation by photodynamic antisense protocol ;(4) Development of live-cell RNA-imaging protocol. Through this research, we are convinced that the nucleic acids with sophisticated functions can reveal unknown functions of" RNA".