Macrocyclic antibiotics production-platform through biosynthetic engineering

在可获得其全基因组信息的有用微生物中，作为具有生产生物活性化合物的高潜力的宿主，开发了用于原核生物的阿维链霉菌和用于真核生物的过氧阿维链霉菌作为基本表达宿主。一种新的控制链霉菌次级代谢产物的激素。在国际上首次分离并鉴定了阿维菌素的结构，同时发现了许多调控基因并鉴定了其功能。名为.因此，开发了一种新的表达宿主，该宿主含有一个新的上调次生代谢的基因，并成功地开发了生物合成基因簇与产物的关联系统。

Among the useful microorganisms on which whole genome information is available, as the host having high potential for the production of bioactive compounds, Streptomyces avermitilis for prokaryotes and As pergillus oryzae for eukaryotes were developed as the basic expression hosts. A novel Streptomyces hormone controlling secondary metabolites in S. avermitilis was isolated and its structure was elucidated for the first time in the world, together with finding many regulatory genes and identification of their functions. With A. oryzae, new expression host harboring a novel gene upregulating secondary metabolism was developed, and system to correlate biosynthetic gene cluster with products was successfully developed.