Analysis of GRWD1, a novel histone-binding protein involved in chromosomal replication initiation and segregation

DNA复制反应的精确调控对基因组信息的完整性至关重要。Cdt1与ORC和CDC6一起将MCM复制解旋酶复合物装载到DNA复制原点。GRWD1是一种新的cdt1结合蛋白。在这个研究项目中，我们得到了以下发现。GRWD1结合Cdc6和Cdt1，从而积累到复制起点。(2) grwd1是一种新的组蛋白结合蛋白，具有组蛋白伴侣子活性。此外，grwd1实际上参与了染色质开放的调控（一般认为，开放的染色质结构促进了染色质上的各种交易）。(3) siRNA沉默GRWD1抑制MCM加载。因此，grwd1可能在调控复制中起着至关重要的作用。

Precise regulation of DNA replication reaction is essential for integrity of genome information. Cdt1, together with ORC and CDC6, loads MCM replication helicase complex onto DNA replication origin. GRWD1 has been identified as a novel Cdt1-binding protein. In this research project, we have obtained following findings.(GRWD1 binds to Cdc6 and Cdt1, thereby accumulates onto replication origins.(2) GRWD1is a novel histone-binding protein and has a histone chaperon activity. Furthermore, GRWD1is actually involvedin regulation of chromatin openness(in general, it is believed thatopen chromatin structure promotes various transactions on chromatin).(3) GRWD1 silencing by siRNA inhibits MCM loading. Therefore, GRWD1may play a crucial role in regulation ofreplication.