Proteins of an entomopoxvirus : its proteomic analysis and a study for the development of technology for the use of its protein fusolin as a synergist of viral insecticides

甲虫痘病毒的基因组首次被完全测序。感染一种鞘翅目动物的病毒，异常铜（anomalala cuprea, AcEPV）的基因组长度约为246 kb，比先前认为的alphaentomopopoxvirus的基因组要小，它由一个以反末端重复序列（ITR）为界的中心编码区组成，这是目前报道的野生型天花病毒中最长的ITR。基因组中有大约250个开放的阅读框。检测到含有丝氨酸蛋白酶抑制剂（serpins）结构域序列的ORF，该结构域可能在哺乳动物而非昆虫中具有免疫功能。将表达病毒增强因子的Fusolin基因引入酵母外源基因表达系统，目的是大量生产该蛋白，以开发病毒杀虫剂增强系统。

The genome of an alphaentomopoxvirus virus was completely sequenced for the first time. The genome of the virus that infects a coleopteran, Anomala cuprea(AcEPV) is ca. 246 kb in length, which is smaller than those of Alphaentomopoxvirus previously considered, and consists of a central coding region bounded by inverted terminal repeats(ITR) that is the longest ITRs among wild type of poxviruses reported. The genome harbors ca. 250 open reading frames. An ORF which contains a serine protease inhibitor(serpins) domain sequence, which may harbor an immunological function in mammalians not insects was detected. Fusolin gene, which expresses a viral enhancing factor, was introduced into an expression system of foreign genes in yeast for the purpose of large amount of the production of the protein for the development of a system for the enhancement of virus insecticides.