Role of neutrophils on diagnosis of the severity in lung dysfunction following hemorrhagic shock

我们通过对四组大鼠出血严重程度的分级，检验了中性粒细胞在诊断肺功能障碍严重程度中的作用。随着出血量的增加，肺组织中肿瘤坏死因子α和白介素1β的表达及肺血清中这两种细胞因子的水平逐渐升高，中性粒细胞的激活频率也随之增加。肺功能障碍指标血清乳酸脱氢酶-3同工酶(LDH-3)、动脉血氧分压(PaO2)和肺泡-动脉血氧分压差(A-ADO2)随出血量增加而加重。失血性休克后5小时肺组织中性粒细胞活化频率与LDH-3水平呈正相关。本研究结果表明，由促炎性细胞因子激活的中性粒细胞向肺内募集的增加与失血性休克后肺功能障碍的进展有定量关系。

We examined the role of neutrophils on diagnosis of the severity in lung dysfunction by grading four groups of severity of bleeding in rats. As the amount of bleeding increased, the expression of mRNA for TNF-α and IL-1β in the lung tissue and the pulmonary serum levels of both cytokines increased progressively up to 5 hours, and the frequency of activated neutrophils increased likewise. The lung dysfunction indices serum lactic dehydrogenase-3 isozyme (LDH-3), partial pressure of arterial oxygen (PaO_2) and alveolar-arterial oxygen tension difference (A-aDO_2) in blood deteriorated as the amount of bleeding increased. The frequency of activated neutrophils in the lung correlated well with the LDH-3 level 5 hours after hemorrhagic shock. The present results demonstrate that the increases of the recruitment of neutrophils into the lung activated by proinflammatory cytokines are quantitatively related to progression of lung dysfunction following hemorrhagic shock.