Regulatory factor on binding of Desmoglein3 and p120ctn during blister formation in pemphigus

为了阐明天疱疮水疱形成的信号通路，我们检测了天疱疮igg处理的角化细胞中pl20octn的磷酸化。(1)我们用天疱疮igg处理的DJM-1细胞进行免疫荧光染色，但未观察到phospho-p120ctn。(2)我们刺激了正常的人角质形成细胞，并对这些细胞进行免疫沉淀。p120ctn的磷酸化在未处理细胞、天疱疮细胞和正常人IgG之间没有显著差异。

In order to elucidate the signal pathway of blister formation in pemphigus, we examined the phosphorylation of pl2Octn in keratinocytes treated with pemphigus-IgG.(1) We performed immunofluorescence staining with DJM-1 cells treated with pemphigus-IgG, however, phospho-p120ctn were not observed.(2) We stimulated normal human keratinocytes and subjected those cells to immunoprecipitation. There are no significant differences in phosphorylation of p120ctn between untreated cells, cells treated with pemphigus and normal human IgG.