The roles of bacterial lipoproteins on bacterial invasion and on intracellular trafficking in host cells

为了研究细菌脂蛋白在细菌入侵宿主细胞中的作用以及对宿主细胞内运输的影响，由于已知一些支原体能够入侵宿主细胞，因此试图纯化支原体脂蛋白。本研究从人支原体中纯化了一种脂蛋白。纯化得到40 kda -脂蛋白，其n端氨基酸序列与P50脂蛋白相同，P50脂蛋白已被报道为人支原体的粘附素。有趣的是，这种脂蛋白可以粘附在THP-1细胞上，但抗TLR2抗体不能抑制这种脂蛋白粘附在THP-1细胞上。我们现在正准备将这些结果提交给国际免疫学杂志。

To study the roles of bacterial lipoproteins on bacterial invasion to host cells and on the effects on intracellular trafficking in host cells, attempts were made to purify a mycoplasmal lipoprotein, since some mycoplasmas are known to be able to invade into host cells. In this study, a lipoprotein was purified from M. hominis. The 40 kDa-lipoprotein was purified, and its N-terminal amino acid sequence was identical to that of P50 lipoprotein, which had already been reported as an adhesin of M. hominis. Interestingly, this lipoprotein could adhere to THP-1 cells but anti TLR2 antibody failed to inhibit adherence of this lipoprotein to THP-1 cells. We are now under preparation for submitting these results to an international journal on immunology.