Molecular and physiological studies on some ectomycorrhizal symbiotic fungi

本研究以日本外生菌根真菌为研究对象，包括松茸口蘑（Tricholoma matsutake）、松茸口蘑（T.bakamatsutake）（不同寄主谱的松茸口蘑同属）、shimeji Lyophylum和shimeji L. decdeces （shimeji L.腐生和系统发育密切相关的物种）。我们用单个担子孢子分离分离了这些真菌的单倍体菌株，并比较了它们的群体生长和外生菌根形成等表型。我们还测试了这些真菌物种对农业感染的能力。最后，我们分别选择了一株具有代表性的单倍体施麦草和一株凋零草。基因组测序采用HiSeq2000系统。

In this study, Japanese ectomycorrhizal fungi, e.g. Tricholoma matsutake， T.bakamatsutake(an allies of T. matsutakewith different host spectrum)，Lyophylum shimejiand L. decastes(saprophytic and phylogenetically closely related species of L. shimeji) were featured. We isolated haploid strains of these fungal species bysingle basidiospore isolations, and compared their phenotypes on colonial growth and ectomycorrhizal formations etc. We also tested competency of these fungal species for Agro-infections. Finally, we chose a representative haploid strain of L. shimejiand L. decastes, respectively. The genomes were sequenced using HiSeq2000 system.