D.I.Y. Medicine:Development of autologous tissue heart valves constructed in the body of the patient

我们利用简单、安全、经济的体内组织工程技术构建了自体组织心脏瓣膜(生物瓣膜)，不需要使用任何特殊的清洁设备，也不需要复杂的程序。将特殊设计的模具植入兔、比格犬和山羊的皮下间隙，用自体组织覆盖4周。脱模后形成三叶瓣状自体组织。它们也被构建在移植到循环系统的管状移植物中。在流路中，观察到在兔体内构建的直径为5 mm的生物瓣的瓣膜运动平稳。其流量模式与家兔主动脉瓣非常接近。在同种异体Beagle动物模型中，成功地将直径10~15 mm的生物瓣膜植入到肺动脉瓣内作为先导实验。最近，它们被组合成金属支架进行导管介入治疗。体内组织工程“生物瓣膜”可能是理想的自体心脏瓣膜假体，有可能取代即将到来的同种异体心脏瓣膜。

We have developed the autologous tissue heart valves (Biovalves) constructed by the simple, safe and economical in vivo tissue engineering without any use of specially clean facilities nor complicated procedures. A specially designed mold embedded in the subcutaneous space of rabbits, beagle dogs and goats were covered by autologous tissues for 4 weeks. After removal of the mold, tri-leaflet valvular shaped autologous tissues were formed. They were also built in the tube grafts for implantation to circulation systems. In the flow circuit, smooth valvular movements of the Biovalves (5 mm in diameter) constructed in the rabbits were observed. Their flow volume pattern was very close to that of native rabbit aortic valves. Biovalves (10-15 mm in diameter) were successfully implanted to the pulmonary valves in the allogeneic beagle model as a pilot experiment. Recently, they were composed to metallic stents for catheter intervention. In vivo tissue engineered “Biovalves” could be idealistic autologous heart valve prostheses with potentiality replacing the short-coming Homografts.