Role of growth cone autonomy and translational trans-acting factors in axon guidance

我们研究了两个mRNA结合的翻译反式作用因子来调节神经元中轴突生长锥体的局部翻译。我们发现，ZBP1是一种基因结合蛋白，它通过脑源性神经营养因子的磷酸化作用，调节β-肌动蛋白mRNA的翻译，进而调节轴突延伸的方向。我们还发现，在生长锥体中存在microRNAs(MiRNAs)，即与mRNA结合的非编码RNAs。我们认为轴突miRNAs可能参与了BDNF调控的轴突伸展。

We have investigated two mRNA-binding, translational trans-acting factors to regulate local translation in axonal growth cones in neurons. We demonstratedthat Zipcode-Binding Protein 1 (ZBP1), an mRNA-binding protein, regulates translation of β-actin mRNA, and then direction of axonal extension, via phosphorylation induced by Brain-Derived Neurotrophic Factor (BDNF). We also found that microRNAs (miRNAs), non-coding RNAs bind to mRNA, exist in growth cones. We indicate the possibility that axonal miRNAs are involved in axonal extension regulated by BDNF.