Effects on the immune response and inhibition of acetylcholine esterase activity by Polygonum hydropiper extracts.

我们描述了加热的蓼提取物（Ayutade和Benitade:TADE）对人体细胞免疫反应和抑制乙酰胆碱酯酶（AChE）的影响。(1)TADE增加了嗜中性粒细胞和单核细胞的吞噬活性和吞噬体-溶酶体融合，并呈剂量依赖性。由于TADE不影响中性粒细胞超氧阴离子的释放，因此TADE成分可能仅影响人体中性粒细胞的某些功能。(2)采用Ellman法定量测定Ayutade和Benitade对AChE的抑制程度。TADE两组分均抑制AChE活性。贝尼塔德水提取物和乙酸乙酯提取物的AChE抑制活性尤为显著。(3)采用含有TADE成分的膳食菜单对10名健康人进行干预检查。比较膳食摄入TADE对认知功能的影响。TADE可能有助于改善和预防痴呆症。

We describe the effects of heated Polygonum hydropiper extracts(Ayutade and Benitade:TADE)on the immune response of human cells and inhibition of acetylcholine esterase(AChE).(1)TADE increased the phagocytic activity and phagosome-lysosome fusion of neutrophils and monocytes in a dose-dependent manner. The TADE components may affect only certain functions of human neutrophils because TADE did not affect superoxide anion release from neutrophils.(2)The degree of inhibition of AChE by Ayutade and Benitade was measured quantitatively with the Ellman method. Both components of TADE inhibited AChE activity. The AChE inhibitory activity of the water extract and ethyl acetate extract of Benitade was especially remarkable.(3)An intervention examination(10 healthy persons) of a dietary menu containing TADE components was carried out. The effects of dietary intake of TADE on cognitive function were compared with control dietary intake.TADE may be useful for improving and preventing dementia.