Characterization and functional analysis of collagen XXII

我们已经鉴定并初步鉴定了一种新的胶原蛋白，XXII型，它是Facit胶原蛋白家族的成员。制备重组蛋白，制备抗体，研究其在小鼠、人和斑马鱼细胞和组织中的表达。这种新蛋白质的一个显著特点是其在组织连接处的独特限制表达。它存在于皮肤的肌腱连接和毛囊-真皮连接的基底膜区，也存在于关节软骨表面的纤维外基质、眼的带状纤维和心脏的肌腱索中。有趣的是，在皮肤中，这种表达依赖于头发周期。为了阐明XXII胶原蛋白的功能，我们建立了基因敲除小鼠，并用吗啉寡核苷酸产生斑马鱼胚胎的病理表型。在这里，我们提出了更多的功能特性的XXII胶原蛋白，并阐明其参与病理过程。在几个器官中的限制性组织表达预测了XXII胶原在涉及许多组织的遗传性或获得性人类综合征中的作用。

We have identified and initially characterized a novel collagen, type XXII, a member of the FACIT collagen family. Collagen XXII was produced as a recombinant protein, antibodies were prepared and the expression was investigated in murine, human and zebra fish cells and tissues. A striking hallmark of this new protein is its uniquely restricted expression at tissue junctions. It is found in the basement membrane zone of myotendinous junctions and the hair follicle-dermis junction in the skin, but also in the extrafibrillar matrix at the surface of articular cartilage, in the zonula fibers of the eye and the chordae tendinae in the heart. Interestingly, in the skin, the expression is hair cycle- dependent. To elucidate the functions of collagen XXII, generation of knockout mice was initiated and morpholino oligonucleotides were used to generate pathological phenotypes in zebra fish embryos. Here we propose further functional characterization of collagen XXII and elucidation of its involvement in pathological processes. The restricted tissue expression in several organs predicts a role for collagen XXII in inherited or acquired human syndromes involving a number of tissues.