Integrated transcr iptomics for dedifferentiation and acquisitien of multipotency in porcine mature adipocytes and follicular granulosa cells.

我们进行了全球基因表达分析，以阐明成熟脂肪细胞（MAs）和滤泡颗粒细胞（GCs）的去分化机制。通过比较去分化过程中基因的整体表达谱，我们在MAs和gc的去分化过程中寻找表达强度增加或减少的基因的特定生物学功能。去分化后，那些在MAs和GCs中表达强烈的基因，如脂质代谢和生殖系统发育基因的表达显著降低。此外，在MAs和GCs的去分化过程中，与细胞形态、细胞骨架、细胞粘附、血管生成、细胞增殖和组织发育相关的基因上调，而与基因表达调控相关的基因下调。

We conducted global gene expression analyses to clarify the dedifferentiationmechanism of mature adipocytes(MAs) and follicular granulosa cells(GCs). By comparingglobal gene expression profiles during the dedifferentiation process, we searched for particular biological functions in those genes whose expression intensities wereincreased or decreased by dedifferentiation of MAs and GCs. After dedifferentiation,expression of those genes expressed strongly in MAs and GCs, such as genes for lipidmetabolism and reproductive system development, was remarkably decreased. Moreover, in the proeess of dedifferentiation of both MAs and GCs, genes involved in cell morphology,cytoskeleton, cell adhesion, angiogenesis, cell proliferation, and tissue developmentwere upregulated, whereas those involved in the regulation of gene expression weredownregulated.