Ultra high resolution Xray crystallographic study for antibiotic mechanisms of action and resistance

碳青霉烯类抗生素是最重要的抗生素之一，在世界范围内被用作“最后手段”。我们研究了四个主题，它们是基于X射线晶体学的碳青霉烯类对β-内酰胺酶的抑制机制，如下所示。(1)碳青霉烯类对C类β-内酰胺酶的抑制机制。(2)来自洋葱伯克霍尔德菌（PenI）和类鼻疽伯克霍尔德菌（佩纳）的佩纳β-内酰胺酶的晶体结构。(3)佩纳碳青霉烯酶-阿维巴坦复合物的晶体结构。(4)B类β-内酰胺酶的抑制剂筛选。我们用晶体结构展示研究的概述。

Carbapenems are one of the most important antibiotics and are used worldwide as "Last Resort". We studied four subjects, which are the inhibition mechanisms of carbapenems to beta-lactamases on the basis of Xray crystallography, as listed below. (1) Carbapenems inhibition mechanism for class C beta-lactamases. (2) Crystal structures of the PenA beta-lactamases from Burkholderia cepacia(PenI) and Burkholderia pseudomallei(PenA). (3) Crystal structure of PenA carbapenemase-avibactam complex. (4) Inhibitor screening of class B beta-lactamases.We are showing the overview of the research with crystal structures.