Role for γδT lymphocytes in autoimmune bone marrow failure syndrome

我们检测了19例纯红细胞发育不全（PRCA）患者的γδ T细胞受体库，发现PRCA患者的γδ T细胞库与健康供者之间存在差异。我们观察到在PRCA患者中，Vδ1 γδ T细胞增加，Vδ2 T细胞减少。再生障碍性贫血患者的Vδ1 γδ T细胞未见升高。CDR3.1大小分布模式在13名PRCA患者中有9名出现偏态，尽管在10名健康个体中也有7名出现偏态。PRCA患者与健康供者之间CDR3.1大小分布无明显变化。此外，在PRCA患者中没有明显一致的氨基酸基序。Vδ1 T细胞的扩增和Vδ2 T细胞的耗竭是慢性获得性PRCA的独特特征，但Vδ1 T细胞的扩增似乎不是cdr3依赖性选择的结果。我们得出结论，Vδ1 T细胞的克隆扩增不是慢性获得性PRCA的一般特征。

We examined the γδ T cell receptor repertoire in 19 pure red cell aplasia (PRCA) patients and found that there was a difference in γδ T-cell repertoires between PRCA patients and healthy donors. We observed an increase in Vδ1 γδ T cells and a decrease in Vδ2 T cells in PRCA patients. An increase of Vδ1 γδ T cells was not found in aplastic anemia patients. CDR3.1 size distribution patterns were skewed in 9 out of 13 PRCA patients examined, although the skewing was also observed in 7 out of 10 healthy individuals. No significant changes were present in CDR3.1 size distribution between PRCA patients and healthy donors. Moreover, no apparent consensus amino acid motifs were identified in PRCA patients. Expansion of Vδ1 T cells and depletion of Vδ2 T cells are unique features for chronic acquired PRCA but expansion of Vδ1 T cells does not seem to be the consequence of CDR3-dependent selection. We conclude that clonal expansion of Vδ1 T cells is not a general feature for chronic acquired PRCA.