Direct injection treatment for intra-parenchymal spinal glioma

C6是一种起源于大鼠脑内的胶质母细胞瘤细胞。体外用替莫唑胺处理细胞进行量效关系研究，100μM的替莫唑胺对细胞生长有明显抑制作用。接下来，我们评估了100TMZ对大鼠脑实质是否有毒性作用。注射速度控制在0.25μL/分钟以下时，未发现脑实质损伤。先将C6细胞植入双侧腰部皮下，C6细胞移植后3天直接瘤内注射TMZ。作为对照，瘤体内注射生理盐水，而不注射TMZ。注射TMZ的患者肿瘤生长受到抑制。然后，将C6细胞注入右侧大脑皮质下，C6细胞移植后3d，肿瘤内直接注射TMZ，对照组肿瘤内注射生理盐水。与对照组相比，TMZ治疗组肿瘤生长明显减慢。

C6 is a glioblstoma cell originated from brain of a rat. The cells were treated by temozolomide (TMZ) in vitro for dose-response study, and the cell growth was definitely suppressed in 100μM of TMZ. And next, we evaluated that whether 100μM TMZ causes toxic effect or not to rat's brain parenchyma. No brain parenchymal damage was identified in the brain as far as inject-speed was kept in less than 0.25μl/minutes. First, C6 cells were implanted into subcutaneous in bilateral lumbar regions, and TMZ was injected directly into the tumor 3 days after the implantation of C6 cells. As a control, saline solution was injected into the tumor instead of TMZ. Tumor growth was suppressed in TMZ injected cases. And next, C6 cells were injected into the right brain sub-cortex, and TMZ was injected directly into the tumor 3 days after the implantation of C6 cells.Saline solution was injected into the tumor in control rats. Compared with control,TMZ-treated tumor showed much slower growth.