An experimental treatment of soft tissue sarcoma by HIFU (high-intensity focused ultrasound)

高强度聚焦超声（HIFU）治疗为癌症的治疗提供了巨大的希望。观察软组织肉瘤在HIFU治疗后的组织学变化，包括抗肿瘤免疫反应。材料与方法：小鼠皮下注射180肉瘤细胞。注射后大约2周，肿瘤被单次HIFU照射。测量肿瘤直径，观察治疗后肿瘤存活率。切除肿瘤，用TUNEL染色、抗酒石酸酸性磷酸酶（TRAP）染色检测肿瘤相关巨噬细胞，并用免疫组化染色检测CD4和CD8。结果：HIFU组肿瘤体积明显小于对照组，生存率明显高于对照组。HIFU组浸润肿瘤的TUNEL-、TRAP-、CD4-和cd8阳性细胞数量明显增加。结论：HIFU即使单次注射也能诱导肿瘤细胞凋亡和肿瘤内巨噬细胞和淋巴细胞浸润。

High-intensity focused ultrasound (HIFU) therapy offers great promise for the treatment of cancer. The histological changes, including the antitumor immunological response, after HIFU treatment was examined in soft tissue sarcoma. Materials and Methods: Sarcoma 180 cells were injected subcutaneously in mice. Approximately 2 weeks after the injection, the tumor was irradiated by a single shot of HIFU. The tumor diameter was measured and the survival rate was observed after treatment. The tumors were resected, and stained with TUNEL stain, tartrate-resistant acid phosphatase (TRAP) stain to detect tumor-associated macrophages, and immunohistochemical stains for CD4 and CD8. Results: The tumor size in the HIFU group was significantly smaller than the control and survival rate was significantly higher. The numbers of TUNEL-, TRAP-, CD4- and CD8-positive cells infiltrating the tumor were significantly higher in the HIFU group. Conclusion: HIFU, even when administered as a single shot, induces apoptosis of tumor cells and intratumoral infiltration of macrophages and lymphocytes.