Investigation of preventive method for periodontal disease by regulating gingival epithelial cell' s function

由于牙龈上皮细胞层具有机械屏障的功能，因此上皮细胞层的通透性表明其对牙周病原体侵袭的防御能力。E-cadherin和claudin-1是调节上皮通透性的关键分子。马来酸伊索格拉定（IM）是一种治疗胃溃疡的药物，在体内和体外研究中，它还能调节放线菌聚集杆菌刺激牙龈上皮的趋化因子分泌和E-cadherin的表达。在本研究中，我们进一步研究了炎症条件下IM对牙龈上皮细胞屏障功能的影响。TNF-α诱导claudin-1和E-cadherin的破坏，IM恢复了人牙龈上皮细胞的破坏。提示IM可通过调节E-cadherin和cladin -1恢复TNF-α-诱导的牙龈上皮屏障破坏。

Since gingival epithelial cell layer functions as a mechanical barrier, the permeability of epithelial cell layer indicates the defensive ability against invasion of periodontal pathogens. E-cadherin and claudin-1 are key molecules to regulate permeability of epithelium. Irsogladine maleate(IM) is a medication for gastric ulcer and also regulates chemokine secretion and E-cadherin expression in gingival epithelium stimulated by Aggregatibacter actinomycetemcomitans in vivo and in vitro studies. In this study, we have further investigated the effects of IM on barrier functions of gingival epithelial cells in inflammatory condition. TNF-α induced the disruption of claudin-1 and E-cadherin and IM recovered the disruption in human gingival epithelial cell. These results suggest that IM recovers the TNF-α-induced disruption of gingival epithelial barrier by regulating E-cadherin and claudin-1.