Application of enzymatically gellable gelatin derivative to peritoneal dissemination treatment

我们评估了具有酚羟基部分的明胶衍生物通过辣根过氧化物酶催化的反应可胶凝的可能性，作为用于治疗腹膜播散的抗癌药物的控制释放的候选物。我们的研究表明，水凝胶不适合该目的。因此，我们试图开发新型的可生物降解和原位胶凝的水凝胶。在这项研究中，我们开发的有前途的水凝胶是支链淀粉的衍生物。具有酚羟基结构的支链淀粉衍生物的水溶液可以通过辣根过氧化物酶催化的反应形成凝胶。水凝胶的一个吸引人的特性是在血清存在下的可降解性。此外，我们可以开发一种新的水凝胶过程中触发葡萄糖使用葡萄糖氧化酶提供过氧化氢的水凝胶过程。

We evaluated the possibility of gelatin derivative possessing phenolic hydroxyl moieties gellable via a horseradish peroxidase-catalyzed reaction as a candidate for controlled release of anti-cancer drugs for the treatment of peritoneal dissemination. Our studies revealed that the hydrogel was not suitable for the purpose. Therefore, we attempted to develop novel biodegradable and in situ gellable hydrogels. The promising hydrogel we developed in this study was the derivative of amylopectin. The aqueous solution of the amylopectin derivative possessing phenolic hydroxyl moieties could be gellable through the horseradish peroxidase-catalyzed reaction. An attractive property of the hydrogel was the degradability in the presence of serum. In addition, we could develop a novel hydrogelation process triggered by glucose using glucose oxidase for supplying hydrogen peroxide for the hydrogelation process.