Development of new gene transfer system for small fish

探讨了利用流体动力学方法对斑马鱼和青鳉进行基因治疗的可能性。用GFP质粒进行基因转移的条件设置。我们在肝脏中发现很少的GFP阳性细胞。与输注小鼠模型相比，转基因效率较小。我们建立了鱼肝纤维化模型，用于评价肝纤维化的改善。高脂饮食和四氯化碳不适合肝纤维化的诱导，DEN治疗2个月比较适合肝纤维化的诱导。

The possibility of gene therapy for zebrafish and medaka with hydrodynamic methods was examined. A condition setup for gene transfer was performed with GFP plasmid. We found few GFP positive cells in the liver. Gene transfer efficiency was small compared with the infusion of the mouse models.We established fish live fibrosis model for evaluation of liver fibrosis improvement. We found that high fat diet and carbon tetrachloride is not suitable for liver fibrosis induction and DEN treatment for two months is suitable for liver fibrosis.