Establisment of differentiation method from induced pluripotent stem(iPS) cells to osteoblasts via mesenchymal stem cells

本研究的目的是建立一种诱导间充质干细胞分化为间充质干细胞的方法，进而将间充质干细胞定向分化为成骨细胞，以期更有效地将间充质干细胞分化为成骨细胞。然后，我们通过添加骨诱导培养液检测了间充质干细胞向成骨细胞的分化情况。成骨细胞分化标志物表达增强。此外，在羟基磷灰石等生物陶瓷上培养间充质干细胞后，成骨细胞的这些分化标志物的表达也增加。因此，iPS细胞可以通过间充质干细胞向成骨细胞分化。

The aim of this study is to establish a method to differentiate iPS cells into mesenchymal stem cells, followed by to differentiate mesenchymal stem cells into osteoblasts, in order to efficiently differentiate from iPS cells into osteoblasts.The expressions of the markers of mesenchymal stem cells, were enhanced by addition of TGF-βand retinoic acid. Then, we examined the differentiation of the mesenchymal stem cells into osteoblasts by addition of bone induced medium. The expressions of the differentiation markers of osteoblasts were enhanced. In addition, it was revealed that the expression of these differentiation markers of osteoblasts was also increased when the mesenchymal stem cells were cultured on bioceramics, such as hydroxyapatite. Therefore, it was demonstrated that the iPS cells can differentiate into osteoblasts via the mesenchymal stem cells.