The influence of human gut bacteria on bioavailability of flavonoids (Consortium: Flavonoids and polyphenols)

该项目旨在阐明肠道微生物群在选定膳食黄酮类化合物转化中的作用，并研究肠道细菌如何以及在多大程度上影响其转化。预计从该项目中获得的数据与口服类黄酮摄入的安全问题有关。由于没有无菌（GF）状态的人类受试者，GF大鼠和与人类肠道细菌相关的大鼠（HFA大鼠）将作为模型。选择黄酮类化合物、花青素和烯酰化黄酮类化合物喂给这些大鼠。随后，收集这些动物的血液、尿液和粪便，并通过HPLC、GC和LC-MS技术对应用物质及其降解产物进行分析。GF和HFA大鼠的比较将表明肠道细菌是否以及在多大程度上转化摄入的化合物。将鉴定细菌转化的产物，并确定它们在动物胃肠道中的吸收。

The proposed project is aimed to elucidate the role of the gut microbiota in the conversion of selected dietary flavonoids and to investigate how and to which extent intestinal bacteria influence their. The data expected to emerge from the project are of relevance to safety issues as related to the oral intake of flavonoids. Since human subjects with a germ-free (GF) status are not available, GF rats and rats associated with human gut bacteria (HFA rats) will serve as models. Selected compounds representing flavones, anthocyanidins and prenylated flavonoids will be fed to these rats. Subsequently, blood, urine and feces will be collected from these animals and analyzed by HPLC, GC and LC-MS techniques for the applied substances and their degradation products. The comparison of GF and HFA rats will indicate whether and to which extent intestinal bacteria transform the ingested compounds. The products of the bacterial conversions will be identified and their absorption in the gastrointestinal tract of the animals be determined.