Regulatory mechanism of AMPA receptor dynamics by phospholipase A_2

浦肯野神经元中AMPA型谷氨酸受体亚单位GluR2的运输是调节小脑中长期抑郁（LTD）的关键机制，其有助于某些形式的运动学习和协调。我们已经证明，胞浆磷脂酶A_2α的完全激活和随后的花生四烯酸从膜磷脂中的释放是突触表面GluR2表达持续下降所必需的。为了阐明磷脂酶A_2和花生四烯酸对GluR2动力学的调节机制，我们试图利用笼状化合物的光解来控制局部游离花生四烯酸的释放，内化GluR2运动的可视化和磷脂组成的比较分析。

Trafficking of AMPA-type glutamate receptor subunit GluR2 in Purkinje neurons is a key mechanism to modulate long-term depression(LTD) in the cerebellum, which contributes to certain forms of motor learning and coordination. We have shown that full activation of cytosolic phospholipase A_2αand consequent liberation of arachidonic acid from membrane phospholipids are required for the prolonged decrease in the synaptic-surface expression of GluR2.To elucidate the regulatory mechanism of GluR2 dynamics by phospholipase A_2 and arachidonic acid, we attempted spatial and temporal control of local free arachidonic acid liberation using photolysis of caged compounds, visualization of internalized GluR2 movement, and comparative analysis of phospholipid composition.