Mechanism of dipeptide synthesis mediated by prolyl aminopeptidase

蛋白酶水解蛋白质并释放相应的肽。众所周知，蛋白酶还能进行肽的合成反应。特别是，Ser/Cys取代蛋白酶的合成活性增加。在本研究中，我们发现Ser/Cys修饰的脯氨酸氨基肽酶也能提高脯氨酸二肽的合成活性。此外，我们还建立了酶法合成脯氨酸羟脯氨酸的方法，该方法已引起人们对预防运动综合征的关注。

Protease hydrolyze protein and release corresponding peptides. It is known that protease also exert synthetic reaction of peptide. Especially, Ser/Cys substituted protease shows increased synthetic activity. In this study, we revealed that Ser/Cys modified prolyl aminopeptidase also exert increased synthetic activity of proline containing dipeptides. Furthermore we established the enzymatic synthetic method of prolyl hydroxylproline which has attracted attention to prevent locomotive syndrome.