Establishment of Cre-driver mouse lines by using the Exchangeable Gene Trap Ckones.

胚胎干细胞基因打靶技术已成为小鼠基因组操作的主要技术，大多数蛋白质编码基因产生条件性敲除等位基因。然而，在条件敲除系统中具有重要作用的Cre驱动小鼠品系的种类还不够多，我们开发了可交换基因捕获克隆数据库（Database for the Exchangeable Gene Trap Clones; EGTC）（http：egtc.jp）。在我们的系统中，报告基因beta-geo可以通过Cre介导的重组被交换到任何感兴趣的DNA中，我们已经用EGTC ES细胞产生了300多个Cre驱动ES细胞系。其中，提供了约250个克隆用于制作嵌合小鼠，可建立100多个小鼠品系。然后我们将Cre驱动小鼠与ROSA 26 R小鼠交配，并对具有Cre驱动等位基因的ROSA 26 R小鼠进行X-gal染色。其中，21-T188 SACre小鼠表现出小脑特异性表达模式。

Gene targeting in embryonic stem cells has become the principal technology for manipulation of the mouse genome, most protein coding genes are producing conditional knock out allele. However a variety of Cre driver mouse line, which has an important role about conditional knock out system, is not enough.We have developed Database for the Exchangeable Gene Trap Clones; EGTC (http://egtc.jp). In our system, reporter beta-geo gene can be exchanged into any other DNA of interest through Cre-mediated recombination.We have produced more than 300 Cre driver ES cell lines using EGTC ES cells. Among them, about 250 clones were provided to make chimeric mice, and more than 100 mouse lines could be established. Then we did mating of Cre driver mice with ROSA26R mice, and performed X-gal staining of ROSA26R mice with Cre driver allele. Among them, 21-T188SACre mouse line exhibited the cerebellum specific expression pattern.