Integration of membrane proteins through the endoplasmic reticulum translocon and other organelle in the eukaryotic cells

在这个项目中，我们解决了真核细胞中通过内质网转位进行蛋白质整合以及膜蛋白靶向其他细胞器的问题。我们证明了：疏水序列的膜整合通过转运子可以由核糖体调节。多肽链的运动被链上带正电荷的氨基酸残基所停滞，然后恢复。在自然产生的膜蛋白的膜整合中，可以观察到边缘疏水片段的强制膜整合。细胞膜中的胆固醇对转运子功能具有多效性。

In this project, we have addressed the protein integration through translocon on the endoplasmic reticulum in the eukaryotic cells and the targeting of membrane proteins to the other organelle. We demonstrated the follows; the membrane integration of hydrophobic sequences though translocon can be regulated by ribosomes. The movement of polypeptide chain is stalled by positively charged amino acid residues on the chain and then resumes. The forced membrane integration of marginally hydrophobic segment can be observed in the membrane integration of naturally occurring membrane protein. Cholesterol in the membrane exerts pleiotropic effects on the translocon function.