Regulation of Pseudomonas aeruginosa gene expression under low oxygen tensions

铜绿假单胞菌在包括囊性纤维化（CF）患者在内的一些患者组中引起严重感染。在CF患者的气道中，铜绿假单胞菌遇到低氧张力，导致基因调控/突变，可导致从非粘液样表型到粘液样表型的变化。利用定向突变、基因组学、蛋白质组学和慢性铜绿假单胞菌肺功能动物模型，我们将鉴定和表征铜绿假单胞菌参与好氧/厌氧转换的基因和蛋白质，并通过RT-PCR分析从CF患者分离的铜绿假单胞菌菌株中氧控制基因的表达。这些研究旨在详细描述CF气道中的厌氧/微氧铜绿假单胞菌表型，这可能有助于抗假单胞菌药物的开发。

Pseudomonas aeruginosa causes severe infections in several patient groups including patients with cystic fibrosis (CF). In airways of CF patients, P. aeruginosa encounters low oxygen tensions, leading to gene regulation/mutations which can cause a change from a non-mucoid to an mucoid phenotype. Using directed mutagenesis, genomics, proteomics and an animal model of chronic P. aeruginosa lung function we will identify and characterize P.aeruginosa genes and proteins involved in the aerobic/anaerobic switch and analyse by RT-PCR the expression of oxygen controlled genes in P. aeruginosa strains isolated from CF patients. These investigations aim at a detailed description of the anaerobic/microaerobic P. aeruginosa phenotype in CF airways, which may be helpful for the development of antipseudomonal drugs.