Study on selective cancer therapy using apoptosis induced by sonodynamically activated drug

近年来，我们一直在研究超声波对卟啉类化合物抗肿瘤活性的影响。本研究旨在探讨卟啉化合物声动力学诱导细胞凋亡在肿瘤治疗中的应用。用超声波和卟啉化合物如PorfimerSodium、ATX-70、ATX-S10、NPe 6和DCPH-Na处理的细胞清楚地显示膜起泡和细胞收缩。超声波和卟啉化合物处理后，细胞内可见DNA梯状条带形成和caspase-3激活，而顺序处理则无此现象。此外，卟啉化合物和超声波的组合增强了ESR测量的氮氧生成。声动力学诱导的细胞凋亡，caspase-3的激活，和氮氧化物的产生显着抑制组氨酸。这些发现表明，超声产生的活性氧，如单线态氧是声动力学诱导的细胞凋亡的重要介质。

Recently, we have been studying antitumor activation of porphyrin compounds by ultrasound. The present study aims to the application of sonodynamically inducted apoptosis by porphyrin compounds for cancer treatment. Cells treated with ultrasound and porphyrin sompounds such as Porfimer Sodium, ATX-70, ATX-S10, NPe6 and DCPH-Na clearly showed membrane blebbing and cell shrinkag. Also, DNA ladder formation and caspase-3 activation were pbserved in cells treated with ultrasound and porphyrin compounds but not in order treatments. In addition, the combination of porphyin compounds and ultrasound enhanced nitroxide generation measured by ESR. Sonodynamically induced apoptosis, caspase-3 activation, and nitroxide generation were significantly suppressed by histidine. These findings suggest that ultrasonically generated reactive oxygen species such as singlet oxygen is an important mediator of sonodynamically induced apoptosis.