Comprehensive identification of abnormally spliced RNAs and elucidation of their significance

在Y14(RNAi)和MAG-1(RNAi)动物中，未剪接的tra-2 RNA泄漏到细胞质中，并被细胞质剪接酶IRE-1异常剪接到tra-2s RNA上。由于表达TRA-2S蛋白的转基因动物表现出与Y14(RNAi)动物一样的种系阳性化，所以由tra-2s RNA产生的蛋白与正常的TRA-2蛋白相比具有显性-负性作用。有趣的是，到目前为止，几乎所有被检测的基因都观察到了由于Y14或MAG-1的抑制而导致的未剪接RNA的泄漏。此外，抑制eIF4AIII和SAP130还导致未剪接的RNA泄漏到细胞质中。双RNAi分析表明，Y14与SAP130和另外两个剪接相关因子相互作用。这些结果表明，EJC核心亚单位与Pre-mRNA结合，起到守门人的作用，监视和阻止未剪接RNA的过早输出，从而有助于线虫的mRNA质量控制。

In Y14(RNAi) and mag-1(RNAi) animals, unspliced tra-2 RNA is leaked to the cytoplasm and unusually spliced to tra-2s RNA by the cytoplasmic splicing enzyme IRE-1. The protein produced from tra-2s RNA acts dominant-negatively against normal TRA-2 protein because transgenic animals expressing TRA-2s protein showed germline masculinization like Y14(RNAi) animals. Interestingly, the leakage of unspliced RNA caused by the inhibition of Y14 or MAG-1 was also observed for almost all genes examined so far. Moreover, inhibition of eIF4AIII and SAP130 also caused leakage of unspliced RNA to the cytoplasm. Double RNAi analysis indicated that Y14 interacts with both SAP130 and two other splicing-related factors. These results suggest that the EJC core subunits associate with pre-mRNA and function as the gatekeeper to watch and block premature export of unspliced RNA, thereby contributing to mRNA quality control in C. elegans.