The role of testicular mono-ADP-ribosylation in men with idiopathic infertility

ART3 （adp -核糖基转移酶3）已被确定为人类非阻塞性无精子症（NOA）的易感基因。本研究的目的是揭示睾丸ART3在男性不育症中的病理生理作用的分子和遗传学基础。(1)蛋白质组学分析表明，ART3与多种蛋白直接相互作用，其中一些蛋白在人类睾丸中表达，参与精子发生。(2)日本人群的病例-对照遗传关联研究发现了3个NOA易感位点，其中一个含有一个与art3相互作用蛋白相关的家族基因。通过蛋白质组学和遗传学分析，我们确定了有希望的候选基因，这些基因可能在人类精子发生的art3依赖性调节中发挥作用。

ART3 (ADP-ribosyltransferase 3) have been identified as a susceptibility gene for non-obstructive azoospermia (NOA) in humans. The objective of this study was to reveal a molecular and genetic basis for further understanding of pathophysiological roles of testicular ART3 involved in male infertility. (1) A proteomic analysis indicated that ART3 directly interacted with various proteins, some of which were expressed in human testes, and involved in spermatogenesis. (2) Case-control genetic association studies in Japanese populations demonstrated three NOA susceptibility loci, one of which contained a family gene of the ART3-interacting proteins. Thought the proteomic and genetic analyses, we identified promising candidate genes which might have roles in ART3-dependent regulation of spermatogenesis in humans.