Chemical tools specifc for auxin polar transport

生长素极性转运通过建立生长素的不对称分布来响应环境刺激，从而调节细胞的生长素反应。因此，生长素运输系统在植物发育中起着至关重要的作用。植物生长素的分布受植物细胞膜上的生长素内流载体/转运体的调控。因此，研究生长素在细胞内分布的动态变化对了解生长素转运系统的分子机制具有重要意义。在本研究中，我们开发了生长素梯度可视化和调控的新化学工具，如荧光生长素和笼型生长素。荧光生长素类似物可以可视化生长素的分布，而圈养生长素可以精确调节细胞生长素水平。这两种化学工具都有望对生长素梯度的形成机制进行剖析。

The auxin polar transport establish an asymmetric auxin distribution to respond environmental stimulus and consequently modulate the auxin responses of cells. Therefore, the auxin transport system plays a crucial role in plant development. The distribution of cellular auxin is regulated by auxin influx and efflux carriers/transporters localized in plant cell membrane. Therefore, the dynamic of intracellular auxin distribution are important for the understanding of molecular mechanism for auxin transport system. In this study, we developed the new chemical tools for the visualization and modulation of auxin gradient, such as fluorescent auxin and caged auxin. Fluorescent auxin analogs can visualize the distribution of auxin, and caged auxin can preciously modulate cellular auxin level. Both chemical tools can be promising to dissect the mechanism for the formation of auxin gradient.