Dynamics of organelles and its regulation by membrane trafficking in Drosophila male meiotic division

进行减数分裂的果蝇原代精母细胞具有便于观察胞内结构和研究细胞分裂后期调控机制的优点。我们建立了多色成像系统，使我们能够对细胞器进行减数分裂过程的延时观察。我们对1030个可能参与膜运输的基因进行了耗尽实验。因此，我们确定了COPI和COPII组分所需的细胞内囊泡运输是男性减数分裂中细胞分裂的必要因素。通过介导裂解沟蛋白或脂质向裂解位点的运输，COPI是细胞分裂起始所必需的。另一方面，通过维持收缩环与质膜之间的连接，COPII对于细胞质分裂的进展是必不可少的。然而，成神经细胞幼虫不需要这两种涂层复合物。

Drosophila primary spermatocytes undergoing meiotic division I have advantages that one can easily observe intracellular structures and can examine regulatory mechanism of later stages of cell divisions. We established multi-color imaging system that makes us possible to perform a time-lapse observation of cell organelles as a progression of meiotic divisions. We carried out depletion experiments of 1030 genes that are possibly involved in membrane trafficking. Consequently, we identified COPI and COPII components required for intracellular vesicle transport as essential factors of cytokinesis in male meiosis. The COPI is required for initiation of cytokinesis by mediating transport of cleavage furrow proteins or lipids toward cleavage sites. On the other hand, the COPII is indispensable for a progression of cytokinesis through maintenance of connection between contractile ring and plasma membrane. However, larval neuroblasts neither need both coatomer complexes.