Establishment of the haploid breeding method using kinetochore malfunction lines

在本研究中，我尝试建立了利用着丝点故障系进行单倍体育种的方法。首先，利用拟南芥、烟草和番茄进行转化，获得表达GFP::CENH3（着丝粒特异性组蛋白H3）的植株。接下来，将敲除内部CENH3的RNAi构建物转化到植物中。一些具有这些结构的转化子被分离出来，但在转化子中内部CENH3的表达水平与野生型相同。这些结果表明，RNAi方法不足以达到目的。

In this study, I tried to establish the haploid breeding method using kinetochore malfunction lines. First, plants expressing GFP::CENH3 (centromere specific histone H3) were made by transformations using Arabidopsis, tobacco and tomato for the purpose. Next, RNAi constructs to knock down internal CENH3 were transformed to the plants. Some transformants having these constructs were isolated but expression levels of internal CENH3 in the transformants were as same as these in wild type. These results suggested that RNAi method is not sufficient for the purpose.