Studies on the puberty of red seabream with special reference to gonadotropins

促性腺激素卵泡刺激素(FSH)和黄体生成素(LH)在鱼类青春期发育过程中起着重要作用。我们新建立了测定红鳍金枪鱼FSH的方法，并用该方法阐明了红鳍金枪鱼血浆中FSH浓度的季节变化。此外，通过体内和体外的研究，我们发现促性腺激素释放激素刺激黄体生成素的分泌，但不刺激黄体生成素的分泌。此外，我们还建立了两个稳定的细胞系，分别分泌FSH和LH，并成功地获得了足够数量的重组激素(30-60 mg)，可用于人工性成熟诱导的研究。

The two gonadotropins, follicle stimulating hormone (FSH) and luteinizing hormone (LH), play pivotal roles in the puberty in fish. We have newly established the method for measuring the FSH of the red seabream and elucidated the seasonal changes of FSH concentrations in the plasma of the red seabream with this method. Also, we found that gonadotropin-releasing hormone stimulates LH secretion but does not stimulate FSH secretion from the pituitary in the red seabream through the several in vivo and in vitro studies. In addition, we have established two stable cell lines that secrete FSH and LH of the red seabream, respectively and succeeded in obtaining sufficient amount of recombinant hormones (30 - 60 mg) that can be utilized for the studies for artificial induction of sexual maturation in fish.