Role of associative fiber tracts on sulcal development of the cerebrum in ferrets

将出生后4~90天的雄性和雌性雪貂用4%多聚甲醛溶液灌流，取出脑组织。通过大体解剖观察，利用这些大脑标本阐明了脑沟和脑回出现的时间顺序。用高空间分辨率的7-Tesla磁共振成像从固定的大脑获取T1W-MRI。基于T1W-MRI的最大密度投影(MIP)可以在3D上显示与髓构筑相关的皮质功能区。脑沟内陷的定量分析显示，男性突出的新皮质和弓状皮质沟的后部延伸，在大脑的颞顶枕区有皮质扩张。使用扩散张量成像(DTI)测量雪貂大脑沟和回的皮质下白质的分数各向异性。然而，尚不清楚脑沟发育与皮质下白质束发育的关系。

Male and female ferrets at postnatal days (PDs) 4 to 90 were perfused with 4% paraformaldehyde solution, and the brains were removed. The chronology of sulcal and gyral emergences were clarified using those brain samples by gross anatomic observations. T1W-MRI was further acquired from the fixed brain using a high spatial resolution 7-tesla MRI. T1W-MRI-based maximum intensity projection (MIP) could delineate functional cortical areas related to myeloarchitecure in 3D. Quantitative analysis of the sulcal infolding revealed a male-prominent posterior extension of neocortical and archicortical sulci with cortical expansion in the temporo-parieto-occipital region of the cerebrum. Fractional anisotropy was measured in the subcortical white matter of the sulcal and gyral regions of the ferret cerebra using diffusion tensor imaging (DTI). However, it was unclear a relation of sulcal development with the development of subcortical white matter bundles.