Analysis of the function of CCL1 during respiratory tract infection in C-C motif ligand-1 transgenic mouse

我们使用表面活性蛋白C启动子（SPC-CCL 1 Tg）产生了CCL 1（NCBI：rs 2282691）转基因小鼠，其在肺中过表达CCL 1基因。建立了SPC-CCL 1转基因小鼠卡介苗（Mycobacterium bovis BacillusCalmette Guérin，BCG）感染模型，镜检发现SPC-CCL 1转基因小鼠肺部肉芽肿明显增多。DNA微阵列分析显示，4,569个基因在转基因小鼠肺组织中的表达是野生型小鼠的3倍或1/3。对这些基因的聚类分析显示，在内质网应激和肉芽肿形成中起重要作用的Ern 1在BCG处理的SPC-CCL 1 Tg小鼠的肺中比野生型小鼠的肺中表达上调。与野生型小鼠相比，SPC-CCL 1 Tg小鼠显示出显著的Ern 1基因表达。

We generated the CCL1 (NCBI: rs2282691) transgenic mice using surfactant protein C promoter (SPC-CCL1 Tg), which overexpressed CCL1 gene in the lungs. We developed the Mycobacterium bovis Bacillus Calmette Guérin (BCG) infection models in SPC-CCL1 Tg mice.Microscopic observation revealed that the numbers of granulomas in the lungs were significantly increased in SPC-CCL1 Tg mice. In DNA microarray analyses, 4,569 genes had 3 times and higher or one third and lower expression in the lung of Tg mice compare to that of WT mouse. Hierarchical cluster analysis of those genes revealed that Ern1, that plays important roles in endoplasmic reticulum stress and in granuloma formation, was more upregulated in the lung of BCG-treated SPC-CCL1 Tg mice compared to that of wild type mice.CCL1 overexpression increased the formation of granulomas upon BCG infection in the lungs. SPC-CCL1 Tg mice showed significant gene expression of Ern1 in comparison with wild type mice.