Physiological diversity of cytosolic T3 binding protein.: Relationship between inflammation and low T3 syndrome

1.通过分析启动子中AP-1位点的启动子活性和胰岛素信号转导机制，揭示了Mu-晶体蛋白的表达受启动子中AP-1位点的调控。与AP-1激活相关的信号，如胰岛素信号，可能对Mu-crystallin mRNA的表达有不同的影响。2.为了研究CRYM对T_3反应基因的精确调控，我们通过改变细胞内的T_3浓度来评估T_3反应基因表达的一系列变化。结果表明，C_(YM)的表达增强并维持了细胞内基因的T_3反应，特别是随着细胞外T_3浓度的变化。

1. To elucidate the mechanisms of its expression, we evaluated the promoter transactivity and insulin signaling via the AP-1 site in the promoter.The results indicated that the expression of mu-crystallin was regulated through the AP-1 site in the promoter. The signals related to AP-1 activation, such as insulin signaling may have diverse effects on mu-crystallin mRNA expression. .2.To examine the precise regulation of T3-responsive genes in the presence of CRYM, we evaluated serial alterations of T3-responsive gene expression by changing pericellular T3 concentrations in the media.The results suggest that CRYM expression increases and sustains the T3 responsiveness of genes in cells, especially with alteration of the pericellular T3 concentration.