Establishment of the novel treatment strategy against urothelial carcinoma controlling for the transcription factors

获得了以下五个结果。(1)We E-cadherin、P-cadherin表达与临床病理特征密切相关，Snail表达是独立的预后因素。(2)In CDDP抗性细胞系; T24 PR的细胞毒作用，证实了NVP-BEZ 235可抑制PI 3 K-Akt-mTOR信号，诱导细胞毒作用。(3)By尼古丁暴露后，T24细胞中的pAkt被激活，细胞活力和肿瘤生长增加。(4)In新型NF-κ B抑制剂DHMEQ可抑制T24 PR细胞NF-κ B的活化、细胞活力和肿瘤生长。(5)In在T24 PR细胞中，在DHMEQ与Paclitaxel的组合中观察到显著的抗癌功效。

The following five results were obtained.(1)We identified that the close association between E-, P-cadherin expression and clinico-pathological features and that Snail expression is an independent prognostic factor.(2)In CDDP-resistant cell line; T24PR, we confirmed that PI3K-Akt-mTOR signal was elevated and NVP-BEZ235 could inhibit the PI3K-Akt-mTOR signal and induce the cytotoxic effect. (3)By nicotine exposure, the pAkt was activated and cell viability and tumor growth increased in T24 cells. (4)In the T24PR cell, DHMEQ which is a novel NF-kappaB inhibitor could inhibit the activation of NF-kappaB, the cell viability, and tumor growth.(5)In the T24PR cell, significant anticancer efficacy was observed in the combination of DHMEQ with Paclitaxel.