Establishment of new treatment strategy for diabetic erectile dysfunction

对38例初治的糖尿病勃起功能障碍患者进行IIEF检查，以评价西地那非的治疗效果。西地那非治疗前后比较，EF分、IS分、OF分、SD分、OS分、IIEF总分均有显著改善(P&lt；0.01)。西地那非的成功率为55.3%。然而，一般来说，西地那非治疗老年男性的成功率约为70%-80%，没有任何并发症。因此，为治疗糖尿病勃起功能障碍提供一种新的有效的替代疗法是有必要的。12岁雄性SD大鼠用戊巴比妥钠40 mg/kg腹腔注射麻醉。切除阴茎周围皮肤后，在阴茎脚处插入25g的杆状针头，连接压力传感器监测阴茎海绵体内压力(ICP)。手术切开下腹部，露出海绵窦口。使用精致的不锈钢双极钩电极进行电刺激。刺激参数为1.5 mA，频率20 Hz，脉宽0.2ms，持续时间60s。当刺激开始时，颅内压开始升高，到刺激结束时，颅内压升高，这种现象被认为是具有重复性的。因此，可以建立大鼠勃起活动的定量测量方法。随着糖尿病勃起功能障碍模型的建立，我们将找到治疗糖尿病勃起功能障碍的新方法。

IIEF was examined for the evaluation of the therapeutic effect of sildenafil in 38 patients with diabetic erectile dysfunction as an initial treatment. In comparing before and after treatment with sildenafil, EF score, IS score, OF score, SD score OS score and total IIEF score were significantly improved, respectively (P<0.01). The successful rate of sildenafil was 55.3%. However in general, the successful rate of sildenafil for aging male without any complication is reported about 70-80%. So an novel effective alternative treatment for diabetic erectile dysfunction would be warranted.Twelve-years-old male Sprague- Dawley rat was anesthetized by intraperitoneal injection of the pentobarbital 40mg/kg. After removal of skin surrounding penis, 25G aliform needle was inserted into the crura of the penis and connected with a pressure transducer for monitoring intracavernous pressure (ICP). A lower abdominal incision was made and the cavernous herve was exposed. Electrostimulation was performed using a delicate stainless steel bipolar hook electrode. The stimulus parameters were 1.5mA, 20Hz frequency, pulse width of 0.2ms, and duration 60s. The ICP-rising started soon when stimulation began, and ended with the stimulation-off This phenomenon was admitted with reproducibility. As a result, the quantitative measurement of rat's erectile activity could be established. With the diabetic erectile dysfunction model, we are going to find the new therapeutic modality for diabetic erectile dysfunction.