Establishment of new treatment strategy for diabetic erectile dysfunction

采用IIEF评价西地那非对38例糖尿病性勃起功能障碍患者的疗效。西地那非治疗前后比较，EF评分、IS评分、OF评分、SD评分、OS评分及IIEF总分均明显改善（P<0.01）。西地那非的成功率为55.3%。然而，一般来说，西地那非用于老年男性的成功率约为70- 80%，无任何并发症。本实验采用5岁雄性Sprague-道利大鼠，腹腔注射戊巴比妥钠40 mg/kg，麻醉后，观察其勃起功能的变化。切除阴茎周围皮肤后，将25 G翼状针插入阴茎根部，连接压力传感器监测海绵体内压（ICP）。做下腹部切口，暴露海绵状埃尔韦。使用精密的不锈钢双极钩状电极进行电刺激。刺激参数为1.5mA，频率20 Hz，脉宽0.2ms，持续时间60 s。当刺激开始时，ICP上升很快开始，并在刺激关闭时结束。结果表明，大鼠勃起活动的定量测定是可行的。通过糖尿病性勃起功能障碍模型的建立，我们将为糖尿病性勃起功能障碍的治疗寻找新的途径。

IIEF was examined for the evaluation of the therapeutic effect of sildenafil in 38 patients with diabetic erectile dysfunction as an initial treatment. In comparing before and after treatment with sildenafil, EF score, IS score, OF score, SD score OS score and total IIEF score were significantly improved, respectively (P<0.01). The successful rate of sildenafil was 55.3%. However in general, the successful rate of sildenafil for aging male without any complication is reported about 70-80%. So an novel effective alternative treatment for diabetic erectile dysfunction would be warranted.Twelve-years-old male Sprague- Dawley rat was anesthetized by intraperitoneal injection of the pentobarbital 40mg/kg. After removal of skin surrounding penis, 25G aliform needle was inserted into the crura of the penis and connected with a pressure transducer for monitoring intracavernous pressure (ICP). A lower abdominal incision was made and the cavernous herve was exposed. Electrostimulation was performed using a delicate stainless steel bipolar hook electrode. The stimulus parameters were 1.5mA, 20Hz frequency, pulse width of 0.2ms, and duration 60s. The ICP-rising started soon when stimulation began, and ended with the stimulation-off This phenomenon was admitted with reproducibility. As a result, the quantitative measurement of rat's erectile activity could be established. With the diabetic erectile dysfunction model, we are going to find the new therapeutic modality for diabetic erectile dysfunction.