Establishment of new treatment strategy for diabetic erectile dysfunction

糖尿病勃起功能障碍治疗新策略的建立

• 批准号: 18591785
• 负责人: KIKUCHI Eiji
• 金额: $ 2.53万
• 依托单位: Keio University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591785/
• 关键词: Erectile Dysfunction; Cavernous Nerve; Intra Cavernous Pressure; 糖尿病性; 勃起不全モデル

IIEF was examined for the evaluation of the therapeutic effect of sildenafil in 38 patients with diabetic erectile dysfunction as an initial treatment. In comparing before and after treatment with sildenafil, EF score, IS score, OF score, SD score OS score and total IIEF score were significantly improved, respectively (P<0.01). The successful rate of sildenafil was 55.3%. However in general, the successful rate of sildenafil for aging male without any complication is reported about 70-80%. So an novel effective alternative treatment for diabetic erectile dysfunction would be warranted.Twelve-years-old male Sprague- Dawley rat was anesthetized by intraperitoneal injection of the pentobarbital 40mg/kg. After removal of skin surrounding penis, 25G aliform needle was inserted into the crura of the penis and connected with a pressure transducer for monitoring intracavernous pressure (ICP). A lower abdominal incision was made and the cavernous herve was exposed. Electrostimulation was performed using a delicate stainless steel bipolar hook electrode. The stimulus parameters were 1.5mA, 20Hz frequency, pulse width of 0.2ms, and duration 60s. The ICP-rising started soon when stimulation began, and ended with the stimulation-off This phenomenon was admitted with reproducibility. As a result, the quantitative measurement of rat's erectile activity could be established. With the diabetic erectile dysfunction model, we are going to find the new therapeutic modality for diabetic erectile dysfunction.

采用IIEF评价西地那非作为初始治疗对38例糖尿病性勃起功能障碍患者的治疗效果。与西地那非治疗前后比较，EF评分、IS评分、OF评分、SD评分、OS评分和IIEF总分均有显著提高（P<0.01）。西地那非的成功率为55.3%。但一般情况下，西地那非治疗老年男性的成功率约为70-80%，无任何并发症。因此，一种新的有效的治疗糖尿病勃起功能障碍的替代方法是有必要的。采用戊巴比妥40mg/kg腹腔注射麻醉12岁雄性Sprague- Dawley大鼠。去除阴茎周围皮肤后，25G异型针插入阴茎脚，连接压力传感器监测海绵内压力（ICP）。下腹部切开，显露海绵状静脉。电刺激使用精致的不锈钢双极钩电极进行。刺激参数为1.5mA，频率20Hz，脉宽0.2ms，持续时间60s。刺激开始时icp上升很快，刺激结束时icp上升很快，这种现象具有可重复性。从而建立了大鼠勃起活动的定量测量方法。以糖尿病勃起功能障碍模型为基础，探索糖尿病勃起功能障碍的治疗新模式。