Development of a novel system of the gene therapy for peripheral arterial diseases.

为了开发新的针对外周动脉疾病的靶点基因治疗方法，我们研究了治疗实验中使用的小鼠后肢缺血模型。很明显，不仅在每种缺血模型中都有合适的治疗靶点，而且小鼠肢体血管解剖的细节也没有得到充分的揭示和考虑。因此，通过对小鼠后肢动脉解剖的详细观察，明确了血管网络结构，并验证了最佳治疗部位与模型制作方法的关系。这些观察结果将有助于血管生成疗法的发展，为今后设计肢体缺血的治疗实验提供基础知识。这些结果发表在英文在线期刊(PlosOne)上，并作为论文发表(医学博士)。

To develop novel gene therapies limited in targeted sites for peripheral arterial diseases, we studied the murine hindlimb ischemia model used in the treatment experiment. There it became apparent that not only appropriate therapeutic target sites in each ischemia model, but also details of vascular anatomy in murine lindlimb are not revealed and considered enough. So, clarify the structure of the vascular network, and verified the relationship between the optimal treatment sites and model fabrication method by observing in detail the murine hindlimb arterial anatomy. These observations will contribute to development of angiogenetic therapies as a basic knowledge for designing therapeutic experiment of limb ischemias into the future. These results were published in English on-line journal (PlosOne), and as a thesis (Doctor of Medicine).