Establishment of bone invasion model by oral cancer and effects of NF-kB inhibitor on bone invasion.

核因子- kb （NF-kB）在包括口腔鳞状细胞癌（OSCC）在内的许多癌症中都是组成性激活的。然而，NF-kB促进骨侵袭的细胞机制尚不清楚。因此，我们在体内研究了NF-kB在OSCC骨侵袭中的作用。10例OSCC侵袭骨的免疫组化染色显示，与正常鳞状上皮细胞相比，OSCC中NF-kB的核易位增加。接下来，我们将小鼠OSCC SCCVII细胞注射到C3H/HeN小鼠的咬肌区。NBD肽是一种选择性NF-kB抑制剂，可减少SCCVII细胞对颧骨和下颌骨的破坏，通过抑制成骨细胞和SCCVII细胞中RANKL的表达减少破骨细胞的数量，增加细胞凋亡，抑制SCCVII细胞的增殖。综上所述，我们的数据清楚地表明，抑制NF-kB有助于抑制OSCC对骨的侵袭

Nuclear factor-kB (NF-kB) is constitutively activated in many cancers, including oral squamous cell carcinoma (OSCC). However, the cellular mechanism underlying NF-kB's promotion of bone invasion by OSCC is unclear. Therefore, we investigated the role of NF-kB in bone invasion by OSCC in vivo. Immunohistochemical staining of OSCC invading bone in 10 patients indicated that the nuclear translocation of NF-kB was increased in OSCC compared with normal squamous epithelial cells. We next injected mouse OSCC SCCVII cells into the masseter region of C3H/HeN mice. Treatment with NBD peptide, a selective NF-kB inhibitor, decreased zygoma and mandible destruction by SCCVII cells, reduced number of osteoclasts by inhibiting RANKL expression in osteoblastic cells and SCCVII cells, increased apoptosis and suppressed the proliferation of SCCVII cells. Taken together, our data clearly indicate that inhibition of NF-kB is useful for inhibiting bone invasion by OSCC