Allocation and optimization of whole-cell-catalysts for the bienzymatic synthesis of hydroxycarboxylic acids and hydroxycarboxamides

提出了一种原子效率高，广泛适用的方法，以合成相关（0.1）的双酶级联反应从相应的醛和HCN合成对映纯2-羟基酸。0.5 M)浓度。所涉及的酶是一种氧化腈酶和一种具有相应对映体偏倚的腈酶。与传统工艺相比，这种工艺将有助于精细化学品的可持续生产，因为它提高了产品的产量和对映体纯度，减少了浪费。该项目需要具有所需对映体偏倚的腈酶，在浓双相水-有机反应介质中，在温和的酸性条件下保持活性。由于这些药物的可用性不够，因此将对它们进行筛选和基因工程。在这个项目中，具有基因工程和酶学能力的微生物学小组将与具有广泛生物催化经验的合成有机化学小组密切合作，分享彼此的能力和设施。

It is proposed to develop an atom-efficient, broadly applicable procedure to synthesize enantiopure 2-hydroxyacids from the corresponding aldehydes and HCN in a bienzymatic cascade at synthetically relevant (0.1 . 0.5 M) concentrations. The enzymes involved are an oxynitrilase and a nitrilase with corresponding enantiomeric bias. Such a process will contribute to the sustainable manufacture of fine chemicals on account of the improvement in yield and enantiomeric purity of the products and the reduction in waste compared with the traditional procedures. The project requires nitrilases with the required enantiomeric bias that maintain their activity under mildly acidic conditions in concentrated biphasic aqueous-organicreaction media. As these are not sufficiently available, they will be screening and genetic engineering. In this project a microbiology group with genetic engineering and enzymology capabilities will closely collaborate with a synthetic organic chemistry group with a broad biocatalysis experience, sharing each others competences and facilities.